Evaluation of Antimicrobial Activity of Aspergillus Spp. Secondary Metabolite Against Some of Clinical Bacteria and Fungi

الملخص

      The present study investigates the inhibitory effect of secondary metabolite of fungus   Aspergillus paraciticus, Aspergillus flavus, and Aspergillus oryzae against four pathogenic bacteria (Escherichia coli, Pseudomonas aeruginosa, Staphylococcus epidermids, and Staphylococcus aureus), and three clinical candida species (C. albicans, C.famata  and  C. guillermondii ) by well diffusion method . The results showed that the secondary metabolite of species  Aspergillus  oryzae has clear inhibitory activity against subjected  bacterial isolates and candida species in the concentrations of( 50 ,100 ,150 )mg/  ml, as compared with the antibiotics amoxicillin and nystatin. There is reverse relationship between the concentration of secondary metabolite and microbial growth. Bacterial pathogens are more susceptible to fungal secondary metabolite than candida species.  Aspergillus paraciticus and Aspergillus flavus secondary metabolite have slight inhibitory effect on pathogenic bacteria in the concentration more than 200mg/ml, but they don't have any inhibitory effects against subjected Candida. In conclusion, present study showed that secondary metabolite of Aspergillus oryzae have antimicrobial activity against various pathogenic bacteria and fungi.